CD272 (BTLA) Mouse Monoclonal Antibody [Clone ID: OTI2E4]

CAT#: CF505688

Carrier-free (BSA/glycerol-free) BTLA mouse monoclonal antibody,clone OTI2E4

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTLA (RC219458, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTLA. Positive lysates LY403628 (100ug) and LC403628 (20ug) can be purchased separately from OriGene.

• 

  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-BTLA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505688)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-BTLA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505688)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-BTLA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505688)

• 

  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-BTLA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505688)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-BTLA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505688)

• Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-BTLA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505688)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-BTLA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505688)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-BTLA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505688)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-BTLA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505688)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-BTLA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505688)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-BTLA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505688)

• Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-BTLA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505688)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-BTLA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505688)

• Flow cytometric analysis of live Sup-B15 cells, using anti-BTLA antibody (TA505688 at 1:100, Red), compared to IgG1 isotype control (Black).

Show all 15 images

Specifications

Product Data

• Clone Name: OTI2E4
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:4000, IHC 1:150, FC 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human BTLA(NP_861445) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 32.6 kDa
• Gene Name: Homo sapiens B and T lymphocyte associated (BTLA), transcript variant 1, mRNA.
• Database Link:
  NP_861445
  Entrez Gene 151888 Human
  UniProt ID Q7Z6A9
• Background: This gene encodes a member of the immunoglobulin superfamily. The encoded protein contains a single immunoglobulin (Ig) domain and is a receptor that relays inhibitory signals to suppress the immune response. Alternative splicing results in multiple transcript variants. Polymorphisms in this gene have been associated with an increased risk of rheumatoid arthritis. [provided by RefSeq, Aug 2011]
• Synonyms: BTLA1; CD272

Reference Data

• Protein Families: Transmembrane