p16 ARC (ARPC5) Mouse Monoclonal Antibody [Clone ID: OTI2G1]

CAT#: CF505696

Carrier-free (BSA/glycerol-free) ARPC5 mouse monoclonal antibody,clone OTI2G1

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARPC5 (RC212631, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARPC5. Positive lysates LY417115 (100ug) and LC417115 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 4 different cell lines by using anti-ARPC5 monoclonal antibodyat 1:200 dilution.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ARPC5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505696)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ARPC5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505696)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-ARPC5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505696)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ARPC5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505696)

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Specifications

Product Data

• Clone Name: OTI2G1
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ARPC5 (NP_005708) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 16.1 kDa
• Gene Name: Homo sapiens actin related protein 2/3 complex subunit 5 (ARPC5), transcript variant 1, mRNA.
• Database Link:
  NP_005708
  Entrez Gene 67771 MouseEntrez Gene 360854 RatEntrez Gene 10092 Human
  UniProt ID O15511
• Background: This gene encodes one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein encoded by this gene, the p16 subunit, has yet to be determined. Alternatively spliced transcript variants encoding different isoforms have been observed for this gene. [provided by RefSeq, Jul
• Synonyms: ARC16; dJ127C7.3; p16-Arc

Reference Data

• Protein Pathways: Fc gamma R-mediated phagocytosis, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton