BBOX1 Mouse Monoclonal Antibody [Clone ID: OTI1E11]

CAT#: CF505731

Carrier-free (BSA/glycerol-free) BBOX1 mouse monoclonal antibody, clone OTI1E11 (formerly 1E11)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BBOX1 (RC203156, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BBOX1. Positive lysates LY418299 (100ug) and LC418299 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-BBOX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505731)

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  Anti-BBOX1 mouse monoclonal antibody (TA505731) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BBOX1 (RC203156).

Specifications

Product Data

• Clone Name: OTI1E11
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:4000, IHC 1:150, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human BBOX1(NP_003977) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 44.5 kDa
• Gene Name: Homo sapiens gamma-butyrobetaine hydroxylase 1 (BBOX1), mRNA.
• Database Link:
  NP_003977
  Entrez Gene 170442 MouseEntrez Gene 8424 Human
  UniProt ID O75936
• Background: This gene encodes gamma butyrobetaine hydroxylase which catalyzes the formation of L-carnitine from gamma-butyrobetaine, the last step in the L-carnitine biosynthetic pathway. Carnitine is essential for the transport of activated fatty acids across the mitochondrial membrane during mitochondrial beta-oxidation. [provided by RefSeq, Jul 2008]
• Synonyms: BBH; BBOX; G-BBH; gamma-BBH

Reference Data

• Protein Pathways: Lysine degradation