Synaptotagmin 4 (SYT4) Mouse Monoclonal Antibody [Clone ID: OTI4H3]

CAT#: CF505862

Carrier-free (BSA/glycerol-free) SYT4 mouse monoclonal antibody, clone OTI4H3 (formerly 4H3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SYT4 (RC206304, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SYT4. Positive lysates LY412338 (100ug) and LC412338 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SYT4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505862)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SYT4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505862)

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  Anti-SYT4 mouse monoclonal antibody (TA505862) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SYT4 (RC206304).

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  Immunofluorescent staining of HeLa cells using anti-SYT4 mouse monoclonal antibody (TA505862).

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Specifications

Product Data

• Clone Name: OTI4H3
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:4000, IHC 1:150, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protei of human SYT4(NP_065834) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 47.8 kDa
• Gene Name: Homo sapiens synaptotagmin 4 (SYT4), mRNA.
• Database Link:
  NP_065834
  Entrez Gene 6860 Human
  UniProt ID Q9H2B2
• Synonyms: HsT1192

Reference Data

• Protein Families: Secreted Protein, Transmembrane