RNF39 Mouse Monoclonal Antibody [Clone ID: OTI5E10]

CAT#: CF505912

Carrier-free (BSA/glycerol-free) RNF39 mouse monoclonal antibody, clone OTI5E10 (formerly 5E10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RNF39 (RC219332, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RNF39. Positive lysates LY410747 (100ug) and LC410747 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-RNF39 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-RNF39 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505912)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RNF39 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505912)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-RNF39 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505912)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-RNF39 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505912)

• Anti-RNF39 mouse monoclonal antibody (TA505912) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RNF39 (RC219332).

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Specifications

Product Data

• Clone Name: OTI5E10
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:200, IHC 1:150, IF 1:100
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human RNF39(NP_079512) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 45.3 kDa
• Gene Name: Homo sapiens ring finger protein 39 (RNF39), transcript variant 1, mRNA.
• Database Link:
  NP_079512
  Entrez Gene 171387 RatEntrez Gene 386454 MouseEntrez Gene 488344 DogEntrez Gene 712215 MonkeyEntrez Gene 80352 Human
  UniProt ID Q9H2S5
• Synonyms: HZF; HZFW; LIRF

Reference Data

• Protein Families: Druggable Genome