RFX3 Mouse Monoclonal Antibody [Clone ID: OTI3F7]

CAT#: CF505916

Carrier-free (BSA/glycerol-free) RFX3 mouse monoclonal antibody,clone OTI3F7

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFX3 (RC201137, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RFX3. Positive lysates LY403344 (100ug) and LC403344 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI3F7
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human RFX3 (NP_602304) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 83.3 kDa
• Gene Name: Homo sapiens regulatory factor X3 (RFX3), transcript variant 2, mRNA.
• Database Link:
  NP_602304
  Entrez Gene 19726 MouseEntrez Gene 5991 Human
  UniProt ID P48380
• Background: This gene is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X1, X2, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with other RFX family members. Two transcript variants encoding different isoforms have been described for this gene, and at least one of the variants utilizes alternative polyadenylation signals. [provided by RefSeq, Jul
• Synonyms: bA32F11.1

Reference Data

• Protein Families: Transcription Factors