TRIM38 Mouse Monoclonal Antibody [Clone ID: OTI16D9]

CAT#: CF505982

Carrier-free (BSA/glycerol-free) TRIM38 mouse monoclonal antibody, clone OTI16D9 (formerly 16D9)

Product Images

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TRIM38 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

• 

  Negative control HEK293T lysate (Left lane) or HEK293T lysate containing recombinant protein fragment for human TRIM38 (NP_006346) gene (amino acids 1-265) (Right lane). Equivalent amounts (5 ug per lane) were separated by SDS-PAGE and then immunoblotted with anti-TRIM38.

• 

  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TRIM38 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505982)

• 

  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-TRIM38 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505982)

• 

  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-TRIM38 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505982)

Show all 5 images

Specifications

Product Data

• Clone Name: OTI16D9
• Applications: IHC, WB
• Recommended Dilution: WB 1:200~4000, IHC 1:150
• Reactivities: Human, Monkey
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-265 of human TRIM38 (NP_006346) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 53.2 kDa
• Gene Name: Homo sapiens tripartite motif containing 38 (TRIM38), mRNA.
• Database Link:
  NP_006346
  Entrez Gene 694861 MonkeyEntrez Gene 10475 Human
  UniProt ID O00635
• Background: This gene encodes a member of the tripartite motif (TRIM) family. The encoded protein contains a RING-type zinc finger, B box-type zinc finger and SPRY domain. The function of this protein has not been identified. A pseudogene of this gene is located on the long arm of chromosome 4. [provided by RefSeq, Jul 2012]
• Synonyms: RNF15; RORET

Reference Data

• Protein Families: Druggable Genome