Junctional Adhesion Molecule 1 (F11R) Mouse Monoclonal Antibody [Clone ID: OTI3H3]

CAT#: CF506017

Carrier-free (BSA/glycerol-free) F11R mouse monoclonal antibody, clone OTI3H3 (formerly 3H3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY F11R (RC200004, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-F11R. Positive lysates LY403392 (100ug) and LC403392 (20ug) can be purchased separately from OriGene.

• 

  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-F11R mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506017)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-F11R mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506017)

• 

  Anti-F11R mouse monoclonal antibody (TA506017) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY F11R (RC200004).

Specifications

Product Data

• Clone Name: OTI3H3
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:4000, IHC 1:150, IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human F11R(NP_653087) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 29.8 kDa
• Gene Name: Homo sapiens F11 receptor (F11R), transcript variant 5
• Database Link:
  NP_653087
  Entrez Gene 50848 Human
  UniProt ID Q9Y624
• Synonyms: CD321; JAM; JAM-1; JAM-A; JAM1; JAMA; JCAM; KAT; PAM-1

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), Epithelial cell signaling in Helicobacter pylori infection, Leukocyte transendothelial migration, Tight junction