MelanA (MLANA) Mouse Monoclonal Antibody [Clone ID: OTI3E2]

CAT#: CF506018

Carrier-free (BSA/glycerol-free) MLANA mouse monoclonal antibody, clone OTI3E2 (formerly 3E2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MLANA (RC204190, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MLANA (1:500)

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  Western blot analysis of extracts (10ug) from 1 cell line by using anti-MLANA monoclonal antibody (1:200).

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-MLANA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506018)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MLANA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506018)

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  Anti-MLANA mouse monoclonal antibody (TA506018) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MLANA (RC204190).

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Specifications

Product Data

• Clone Name: OTI3E2
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:4000, IHC 1:150, IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MLANA(NP_005502) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 13 kDa
• Gene Name: Homo sapiens melan-A (MLANA), mRNA.
• Database Link:
  NP_005502
  Entrez Gene 2315 Human
  UniProt ID Q16655
• Synonyms: MART-1; MART1

Reference Data

• Protein Families: Transmembrane