Junctional Adhesion Molecule 1 (F11R) Mouse Monoclonal Antibody [Clone ID: OTI6E11]

CAT#: CF506034

Carrier-free (BSA/glycerol-free) F11R mouse monoclonal antibody, clone OTI6E11 (formerly 6E11)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY F11R (RC200004, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-F11R. Positive lysates LY403392 (100ug) and LC403392 (20ug) can be purchased separately from OriGene.

• 

  Anti-F11R mouse monoclonal antibody (TA506034) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY F11R (RC200004).

Specifications

Product Data

• Clone Name: OTI6E11
• Applications: IF, WB
• Recommended Dilution: WB 1:4000, IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human F11R(NP_653087) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 29.8 kDa
• Gene Name: Homo sapiens F11 receptor (F11R), transcript variant 5
• Database Link:
  NP_653087
  Entrez Gene 50848 Human
  UniProt ID Q9Y624
• Background: Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is an important regulator of tight junction assembly in epithelia. In addition, the encoded protein can act as (1) a receptor for reovirus, (2) a ligand for the integrin LFA1, involved in leukocyte transmigration, and (3) a platelet receptor. Multiple 5' alternatively spliced variants, encoding the same protein, have been identified but their biological validity has not been established. [provided by RefSeq, Jul 2008]
• Synonyms: CD321; JAM; JAM-1; JAM-A; JAM1; JAMA; JCAM; KAT; PAM-1

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), Epithelial cell signaling in Helicobacter pylori infection, Leukocyte transendothelial migration, Tight junction