CD3E Mouse Monoclonal Antibody [Clone ID: OTI3E10]

CAT#: CF506064

Carrier-free (BSA/glycerol-free) CD3E mouse monoclonal antibody,clone OTI3E10

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CD3E (Cat# RC208276, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD3E(Cat# TA506064). Positive lysates LY400242 (100ug) and LC400242 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD3E monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Western blot analysis of extracts (10ug) from 8 Human tissue by using anti-CD3E monoclonal antibody at 1:200 (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: Colon).

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-CD3E mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, TA506064) (1:2000) (1:4000)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CD3E mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, TA506064) (1:2000) (1:4000)

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-CD3E mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, TA506064) (1:2000) (1:4000)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD3E mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, TA506064) (1:2000) (1:4000)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD3E mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506064)

• Anti-CD3E mouse monoclonal antibody (TA506064) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD3E (RC208276).

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Specifications

Product Data

• Clone Name: OTI3E10
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:200~4000, IHC 1:150, IF 1:100
• Reactivities: Human, Dog, Monkey
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CD3E(NP_000724) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 20.7 kDa
• Gene Name: CD3e molecule
• Database Link:
  NP_000724
  Entrez Gene 442981 DogEntrez Gene 699467 MonkeyEntrez Gene 916 Human
  UniProt ID P07766
• Background: The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]
• Synonyms: IMD18; T3E; TCRE

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Hematopoietic cell lineage, Primary immunodeficiency, T cell receptor signaling pathway