Synaptophysin (SYP) Mouse Monoclonal Antibody [Clone ID: OTI1G3]

CAT#: CF506068

Carrier-free (BSA/glycerol-free) SYP mouse monoclonal antibody, clone OTI1G3 (formerly 1G3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SYP (RC208972, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SYP. Positive lysates LY401102 (100ug) and LC401102 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-SYP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506068)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SYP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506068)

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  Anti-SYP mouse monoclonal antibody (TA506068) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SYP (RC208972).

Specifications

Product Data

• Clone Name: OTI1G3
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:150, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SYP(NP_003170) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 33.7 kDa
• Gene Name: Homo sapiens synaptophysin (SYP), mRNA.
• Database Link:
  NP_003170
  Entrez Gene 20977 MouseEntrez Gene 24804 RatEntrez Gene 6855 Human
  UniProt ID P08247
• Background: This gene encodes an integral membrane protein of small synaptic vesicles in brain and endocrine cells. The protein also binds cholesterol and is thought to direct targeting of vesicle-associated membrane protein 2 (synaptobrevin) to intracellular compartments. Mutations in this gene are associated with X-linked mental retardation (XLMR). [provided by RefSeq, Aug
• Synonyms: MRX96; MRXSYP

Reference Data

• Protein Families: Secreted Protein, Transmembrane