SAE1 Mouse Monoclonal Antibody [Clone ID: OTI1G8]

CAT#: CF506071

Carrier-free (BSA/glycerol-free) SAE1 mouse monoclonal antibody, clone OTI1G8 (formerly 1G8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAE1 (RC201820, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SAE1. Positive lysates LY401688 (100ug) and LC401688 (20ug) can be purchased separately from OriGene.

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SAE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506071)

• 

  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SAE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506071)

• 

  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-SAE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506071)

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SAE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506071)

• 

  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SAE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506071)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-SAE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506071)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-SAE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506071)

• Anti-SAE1 mouse monoclonal antibody (TA506071) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SAE1 (RC201820).

• Immunofluorescent staining of HeLa cells using anti-SAE1 mouse monoclonal antibody (TA506071).

Show all 10 images

Specifications

Product Data

• Clone Name: OTI1G8
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:4000, IHC 1:150, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SAE1(NP_005491) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 38.3 kDa
• Gene Name: Homo sapiens SUMO1 activating enzyme subunit 1 (SAE1), transcript variant 1, mRNA.
• Database Link:
  NP_005491
  Entrez Gene 56459 MouseEntrez Gene 308384 RatEntrez Gene 10055 Human
  UniProt ID Q9UBE0
• Background: Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]). [supplied by OMIM, Mar 2010]
• Synonyms: AOS1; HSPC140; SUA1; UBLE1A

Reference Data

• Protein Pathways: Ubiquitin mediated proteolysis