Chromogranin A (CHGA) Mouse Monoclonal Antibody [Clone ID: OTI2E12]

CAT#: CF506098

Carrier-free (BSA/glycerol-free) CHGA mouse monoclonal antibody,clone OTI2E12

Product Images

Anti-CHGA mouse monoclonal antibody (TA506098) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CHGA (RC200492).

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CHGA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506098)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CHGA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506098)

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHGA (RC200492, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHGA. Positive lysates LY400511 (100ug) and LC400511 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CHGA monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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Specifications

Product Data

• Clone Name: OTI2E12
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:200~4000, IHC 1:150, IF 1:100
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CHGA(NP_001266) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 48.9 kDa
• Gene Name: Homo sapiens chromogranin A (CHGA), transcript variant 1, mRNA.
• Database Link:
  NP_001266
  Entrez Gene 12652 MouseEntrez Gene 1113 Human
  UniProt ID P10645
• Background: The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Other peptides, including chromostatin, beta-granin, WE-14 and GE-25, are also derived from the full-length protein. However, biological activities for these molecules have not been shown. [provided by RefSeq, Jul 2008]
• Synonyms: CGA

Reference Data

• Protein Families: Druggable Genome, Secreted Protein