ERG Mouse Monoclonal Antibody [Clone ID: OTI2G8]

CAT#: CF506107

Carrier-free (BSA/glycerol-free) ERG mouse monoclonal antibody,clone OTI2G8

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERG (RC208093, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERG. Positive lysates LY403650 (100ug) and LC403650 (20ug) can be purchased separately from OriGene.

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  Anti-ERG mouse monoclonal antibody (TA506107) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERG (RC208093).

Specifications

Product Data

• Clone Name: OTI2G8
• Applications: IF, WB
• Recommended Dilution: WB 1:4000, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ERG(NP_891548) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 53.7 kDa
• Gene Name: Homo sapiens ETS transcription factor ERG (ERG), transcript variant 1, mRNA.
• Database Link:
  NP_891548
  Entrez Gene 13876 MouseEntrez Gene 170909 RatEntrez Gene 2078 Human
  UniProt ID P11308
• Background: This gene encodes a member of the erythroblast transformation-specific (ETS) family of transcriptions factors. All members of this family are key regulators of embryonic development, cell proliferation, differentiation, angiogenesis, inflammation, and apoptosis. The protein encoded by this gene is mainly expressed in the nucleus. It contains an ETS DNA-binding domain and a PNT (pointed) domain which is implicated in the self-association of chimeric oncoproteins. This protein is required for platelet adhesion to the subendothelium, inducing vascular cell remodeling. It also regulates hematopoesis, and the differentiation and maturation of megakaryocytic cells. This gene is involved in chromosomal translocations, resulting in different fusion gene products, such as TMPSSR2-ERG and NDRG1-ERG in prostate cancer, EWS-ERG in Ewing's sarcoma and FUS-ERG in acute myeloid leukemia. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jan
• Synonyms: erg-3; p55

Reference Data

• Protein Families: Druggable Genome, Transcription Factors