Kallikrein 8 (KLK8) Mouse Monoclonal Antibody [Clone ID: OTI1D2]

CAT#: CF506124

Carrier-free (BSA/glycerol-free) KLK8 mouse monoclonal antibody, clone OTI1D2 (formerly 1D2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KLK8 (RC208152, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KLK8. Positive lysates LY416130 (100ug) and LC416130 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-KLK8 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

• 

  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-KLK8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506124)

• 

  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-KLK8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506124)

• 

  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-KLK8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506124)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-KLK8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506124)

• Anti-KLK8 mouse monoclonal antibody (TA506124) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KLK8 (RC208152).

Show all 7 images

Specifications

Product Data

• Clone Name: OTI1D2
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:4000, IHC 1:150, IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human KLK8(NP_009127) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 27.9 kDa
• Gene Name: Homo sapiens kallikrein related peptidase 8 (KLK8), transcript variant 1, mRNA.
• Database Link:
  NP_009127
  Entrez Gene 11202 Human
  UniProt ID O60259
• Background: Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Alternate splicing of this gene results in four transcript variants encoding four different isoforms. The isoforms exhibit distinct patterns of expression that suggest roles in brain plasticity and ovarian cancer. [provided by RefSeq, Jul
• Synonyms: HNP; NP; NRPN; PRSS19; TADG14

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Transmembrane