LMO2 Mouse Monoclonal Antibody [Clone ID: OTI1C8]

CAT#: CF506139

Carrier-free (BSA/glycerol-free) LMO2 mouse monoclonal antibody, clone OTI1C8 (formerly 1C8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LMO2 (RC205376, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LMO2. Positive lysates LY432149 (100ug) and LC432149 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-LMO2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506139)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-LMO2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506139)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-LMO2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506139)

Specifications

Product Data

• Clone Name: OTI1C8
• Applications: IHC, WB
• Recommended Dilution: WB 1:4000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human LMO2(NP_005565) produced in HEk293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 18.2 kDa
• Gene Name: Homo sapiens LIM domain only 2 (LMO2), transcript variant 1, mRNA.
• Database Link:
  NP_005565
  Entrez Gene 16909 MouseEntrez Gene 362176 RatEntrez Gene 4005 Human
  UniProt ID P25791
• Background: LMO2 encodes a cysteine-rich, two LIM-domain protein that is required for yolk sac erythropoiesis. The LMO2 protein has a central and crucial role in hematopoietic development and is highly conserved. The LMO2 transcription start site is located approximately 25 kb downstream from the 11p13 T-cell translocation cluster (11p13 ttc), where a number T-cell acute lymphoblastic leukemia-specific translocations occur. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2008]
• Synonyms: RBTN2; RBTNL1; RHOM2; TTG2

Reference Data

• Protein Families: Druggable Genome