DDX58 Mouse Monoclonal Antibody [Clone ID: OTI6C1]

CAT#: CF506141

Carrier-free (BSA/glycerol-free) DDX58 mouse monoclonal antibody, clone OTI6C1 (formerly 6C1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DDX58 (RC217615, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DDX58. Positive lysates LY415370 (100ug) and LC415370 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 1 cell line by using anti-DDX58 monoclonal antibody (1:200).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type A549 cells and DDX58-Knockout A549 cells (KO, Cat# LC806244) were separated by SDS-PAGE and immunoblotted with anti-DDX58 monoclonal antibody TA506141 (1:500). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-DDX58 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506141)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-DDX58 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506141)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-DDX58 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506141)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-DDX58 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506141)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-DDX58 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506141)

• Anti-DDX58 mouse monoclonal antibody (TA506141) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DDX58 (RC217615).

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Specifications

Product Data

• Clone Name: OTI6C1
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:4000, IHC 1:150, IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human DDX58(NP_055129) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 106.4 kDa
• Gene Name: Homo sapiens DExD/H-box helicase 58 (DDX58), mRNA.
• Database Link:
  NP_055129
  Entrez Gene 23586 Human
  UniProt ID O95786
• Background: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. This gene encodes a protein containing RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of immune response. [provided by RefSeq, Jul 2008]
• Synonyms: RIG-I; RIGI; RLR-1

Reference Data

• Protein Pathways: Cytosolic DNA-sensing pathway, RIG-I-like receptor signaling pathway