CD105 (ENG) Mouse Monoclonal Antibody [Clone ID: OTI3H5]

CAT#: CF506145

Carrier-free (BSA/glycerol-free) ENG mouse monoclonal antibody, clone OTI3H5 (formerly 3H5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ENG (RC221699, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ENG. Positive lysates LY424919 (100ug) and LC424919 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ENG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506145)

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  Immunofluorescent staining of HeLa cells using anti-ENG mouse monoclonal antibody (TA506145).

Specifications

Product Data

• Clone Name: OTI3H5
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:1000, IHC 1:150, IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ENG(NP_000109) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 65 kDa
• Gene Name: Homo sapiens endoglin (ENG), transcript variant 2, mRNA.
• Database Link:
  NP_000109
  Entrez Gene 2022 Human
  UniProt ID P17813
• Background: This gene encodes a homodimeric transmembrane protein which is a major glycoprotein of the vascular endothelium. This protein is a component of the transforming growth factor beta receptor complex and it binds TGFB1 and TGFB3 with high affinity. Mutations in this gene cause hereditary hemorrhagic telangiectasia, also known as Osler-Rendu-Weber syndrome 1, an autosomal dominant multisystemic vascular dysplasia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]
• Synonyms: END; HHT1; ORW1

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane