ZFP38 (ZSCAN21) Mouse Monoclonal Antibody [Clone ID: OTI3B3]

CAT#: CF506148

Carrier-free (BSA/glycerol-free) ZSCAN21 mouse monoclonal antibody, clone OTI3B3 (formerly 3B3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZSCAN21 (RC208411, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZSCAN21. Positive lysates LY407804 (100ug) and LC407804 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ZSCAN21 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Specifications

Product Data

• Clone Name: OTI3B3
• Applications: WB
• Recommended Dilution: WB 1:200~500
• Reactivities: Human, Monkey, Mouse, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ZSCAN21(NP_666019) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 53.5 kDa
• Gene Name: Homo sapiens zinc finger and SCAN domain containing 21 (ZSCAN21), transcript variant 3, mRNA.
• Database Link:
  NP_666019
  Entrez Gene 22697 MouseEntrez Gene 710501 MonkeyEntrez Gene 7589 Human
  UniProt ID Q9Y5A6
• Synonyms: NY-REN-21; Zipro1; ZNF38

Reference Data

• Protein Families: Transcription Factors