Cytochrome C Oxidase subunit VIc (COX6C) Mouse Monoclonal Antibody [Clone ID: OTI4B11]

CAT#: CF506179

Carrier-free (BSA/glycerol-free) COX6C mouse monoclonal antibody, clone OTI4B11 (formerly 4B11)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY COX6C (RC200374, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-COX6C. Positive lysates LY418028 (100ug) and LC418028 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-COX6C monoclonal antibody (1:200).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-COX6C mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506179)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-COX6C mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506179)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-COX6C mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506179)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-COX6C mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506179)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-COX6C mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506179)

• Anti-COX6C mouse monoclonal antibody (TA506179) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY COX6C (RC200374).

• Immunofluorescent staining of HeLa cells using anti-COX6C mouse monoclonal antibody (TA506179).

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Specifications

Product Data

• Clone Name: OTI4B11
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:4000, IHC 1:150, IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human COX6C(NP_004365) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 8.6 kDa
• Gene Name: Homo sapiens cytochrome c oxidase subunit 6C (COX6C), mRNA.
• Database Link:
  NP_004365
  Entrez Gene 1345 Human
  UniProt ID P09669
• Background: Cytochrome c oxidase, the terminal enzyme of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. It is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may be involved in the regulation and assembly of the complex. This nuclear gene encodes subunit VIc, which has 77% amino acid sequence identity with mouse subunit VIc. This gene is up-regulated in prostate cancer cells. A pseudogene has been found on chromosomes 16p12. [provided by RefSeq, Jul
• Synonyms: cytochrome c oxidase subunit VIc; cytochrome c oxidase subunit VIc preprotein

Reference Data

• Protein Families: Transmembrane
• Protein Pathways: Alzheimer's disease, Cardiac muscle contraction, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease