MSI2 Mouse Monoclonal Antibody [Clone ID: OTI2F10]

CAT#: CF506196

Carrier-free (BSA/glycerol-free) MSI2 mouse monoclonal antibody, clone OTI2F10 (formerly 2F10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MSI2 (RC201003, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MSI2. Positive lysates LY403371 (100ug) and LC403371 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MSI2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MSI2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506196)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MSI2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506196)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-MSI2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506196)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MSI2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506196)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-MSI2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506196)

• Anti-MSI2 mouse monoclonal antibody (TA506196) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MSI2 (RC201003).

• Immunofluorescent staining of HeLa cells using anti-MSI2 mouse monoclonal antibody (TA506196).

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Specifications

Product Data

• Clone Name: OTI2F10
• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:200 - 1:1000, IHC 1:150, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MSI2(NP_620412) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 35 kDa
• Gene Name: Homo sapiens musashi RNA binding protein 2 (MSI2), transcript variant 1, mRNA.
• Database Link:
  NP_620412
  Entrez Gene 76626 MouseEntrez Gene 360596 RatEntrez Gene 124540 Human
  UniProt ID Q96DH6
• Synonyms: MSI2H