Von Hippel Lindau (VHL) Mouse Monoclonal Antibody [Clone ID: OTI1E1]

CAT#: CF506222

Carrier-free (BSA/glycerol-free) VHL mouse monoclonal antibody, clone OTI1E1 (formerly 1E1)

Formulation: Standard Carrier Free


  View other "OTI1E1" antibodies (2)

USD 465.00

In Stock*

Size
    • 100 ug

Specifications

Product Data
Clone Name OTI1E1
Applications IHC, WB
Recommended Dilution WB 1:4000, IHC 1:150
Reactivities Human, Mouse, Rat
Host Mouse
Isotype IgG2b
Clonality Monoclonal
Immunogen Full length human recombinant protein of human VHL(NP_000542) produced in HEK293T cell.
Formulation Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Predicted Protein Size 24 kDa
Gene Name Homo sapiens von Hippel-Lindau tumor suppressor (VHL), transcript variant 1, mRNA.
Background Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to a variety of malignant and benign tumors. A germline mutation of this gene is the basis of familial inheritance of VHL syndrome. The protein encoded by this gene is a component of the protein complex that includes elongin B, elongin C, and cullin-2, and possesses ubiquitin ligase E3 activity. This protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
Synonyms HRCA1; pVHL; RCA1; VHL1
Reference Data
Protein Families Druggable Genome, Transcription Factors
Protein Pathways Pathways in cancer, Renal cell carcinoma, Ubiquitin mediated proteolysis

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