Apc11 (ANAPC11) Mouse Monoclonal Antibody [Clone ID: OTI1A8]

CAT#: CF506235

Carrier-free (BSA/glycerol-free) ANAPC11 mouse monoclonal antibody, clone OTI1A8 (formerly 1A8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANAPC11 (RC200097, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANAPC11. Positive lysates LY424181 (100ug) and LC424181 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ANAPC11 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506235)

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  Anti-ANAPC11 mouse monoclonal antibody (TA506235) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ANAPC11 (RC200097).

Specifications

Product Data

• Clone Name: OTI1A8
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:4000, IHC 1:150, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ANAPC11(NP_001002244) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 20.5 kDa
• Gene Name: Homo sapiens anaphase promoting complex subunit 11 (ANAPC11), transcript variant 1, mRNA.
• Database Link:
  NP_001002244
  Entrez Gene 66156 MouseEntrez Gene 498030 RatEntrez Gene 51529 Human
  UniProt ID Q9NYG5
• Synonyms: APC11; Apc11p; HSPC214

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation, Ubiquitin mediated proteolysis