CD19 Mouse Monoclonal Antibody [Clone ID: OTI3B10]

CAT#: CF506236

Carrier-free (BSA/glycerol-free) CD19 mouse monoclonal antibody, clone OTI3B10 (formerly 3B10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD19 (RC202922, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD19. Positive lysates LY400678 (100ug) and LC400678 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CD19 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, TA506236) (1:600)

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-CD19 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, TA506236) (1:600)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD19 mouse monoclonal antibody. (TA506236; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD19 mouse monoclonal antibody. (TA506236; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

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  Anti-CD19 mouse monoclonal antibody (TA506236) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD19 (RC202922).

• Living HEK293T cells transfected with either CD19 (RC202922) overexpression plasmid or empty vector control plasmid were immunostained by either anti-CD19 antibody (TA506236) or a nonspecific negative control antibody ([TA180143]) and then analyzed by flow cytometry. CD19 (red) or empty vector (blue) transfected cells with anti-CD19 antibody. CD19 (green) or empty vector (aqua) transfected cells with control antibody (1:100).

• Flow cytometric Analysis of living RBC-lysed human peripheral blood cells, using anti-huamn CD19 antibody (TA506236), (Red), compared to a nonspecific negative control antibody (TA180144), (green) and PBS, (blue) (1:20).

• Flow cytometric Analysis of living Raji cells, using anti-CD19 antibody (TA506236), (Red), compared to a nonspecific negative control antibody [TA180143], (green), or PBS (blue) (1:20).

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Specifications

Product Data

• Clone Name: OTI3B10
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:4000, IHC 1:150, IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CD19(NP_001761) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 60.9 kDa
• Gene Name: Homo sapiens CD19 molecule (CD19), transcript variant 2, mRNA.
• Database Link:
  NP_001761
  Entrez Gene 930 Human
  UniProt ID P15391
• Background: Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. [provided by RefSeq, Jul 2008]
• Synonyms: B4; CVID3

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: B cell receptor signaling pathway, Hematopoietic cell lineage, Primary immunodeficiency