CD33 Mouse Monoclonal Antibody [Clone ID: OTI1H8]

CAT#: CF506237

Carrier-free (BSA/glycerol-free) CD33 mouse monoclonal antibody, clone OTI1H8 (formerly 1H8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD33 (RC207023, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD33 (1:500).

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD33 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA506237) (1:500)

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  Flow cytometric analysis of living 293T cells transfected with CD33 overexpression plasmid (RC207023), Red)/empty vector (PS100001, Blue) using anti-CD33 antibody (TA506237). Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).

Specifications

Product Data

• Clone Name: OTI1H8
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:4000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CD33(NP_001763) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 38 kDa
• Gene Name: Homo sapiens CD33 molecule (CD33), transcript variant 1, mRNA.
• Database Link:
  NP_001763
  Entrez Gene 945 Human
  UniProt ID P20138
• Synonyms: p67; SIGLEC-3; SIGLEC3

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Hematopoietic cell lineage