CD64 (FCGR1A) Mouse Monoclonal Antibody [Clone ID: OTI1A8]

CAT#: CF506331

Carrier-free (BSA/glycerol-free) FCGR1A mouse monoclonal antibody, clone OTI1A8 (formerly 1A8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FCGR1A (RC207487, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FCGR1A. Positive lysates LY400193 (100ug) and LC400193 (20ug) can be purchased separately from OriGene.

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-FCGR1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506331)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-FCGR1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506331)

• 

  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-FCGR1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506331)

• 

  Immunofluorescent staining of 293T cells transfected by pCMV6-ENTRY FCGR1A (RC207487) using anti-FCGR1A antibody (TA506331/green, upper left; DAPI/blue, lower left; MERGED, upper right). 293T cells transfected with empty vector served as a negative control (MERGED, lower right) (1:100).

• 

  Flow cytometric analysis of living 293T cells transfected with FCGR1A overexpression plasmid (RC207487), Red)/empty vector (PS100001, Blue) using anti-FCGR1A antibody (TA506331). Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).

Show all 6 images

Specifications

Product Data

• Clone Name: OTI1A8
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinanti protein of human FCGR1A(NP_000557) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 42.5 kDa
• Gene Name: Homo sapiens Fc fragment of IgG receptor Ia (FCGR1A), transcript variant 2, mRNA.
• Database Link:
  NP_000557
  Entrez Gene 2209 Human
  UniProt ID P12314
• Background: This gene encodes a protein that plays an important role in the immune response. This protein is a high-affinity Fc-gamma receptor. The gene is one of three related gene family members located on chromosome 1. [provided by RefSeq, Jul 2008]
• Synonyms: CD64; CD64A; FCRI; IGFR1

Reference Data

• Protein Families: Transmembrane
• Protein Pathways: Fc gamma R-mediated phagocytosis, Hematopoietic cell lineage, Systemic lupus erythematosus