CD163 Mouse Monoclonal Antibody [Clone ID: OTI2G12]

CAT#: CF506380

Carrier-free (BSA/glycerol-free) CD163 mouse monoclonal antibody, clone OTI2G12 (formerly 2G12)

Specifications

Product Data

• Clone Name: OTI2G12
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:1000, IHC 1:150, IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CD163(NP_004235) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 125.3 kDa
• Gene Name: CD163 molecule
• Database Link:
  NP_004235
  Entrez Gene 9332 Human
  UniProt ID Q86VB7
• Background: The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]
• Synonyms: M130; MM130; SCARI1

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Transmembrane