CD163 Mouse Monoclonal Antibody [Clone ID: OTI3C4]

CAT#: CF506389

Carrier-free (BSA/glycerol-free) CD163 mouse monoclonal antibody, clone OTI3C4 (formerly 3C4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD163 (RC208557, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD163. Positive lysates LY401362 (100ug) and LC401362 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-CD163 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506389)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD163 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA506389) (1:500)

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  Anti-CD163 mouse monoclonal antibody (TA506389) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD163 (RC208557).

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  CD163 Luminex with 3C1 Capture (TA506393) and 3C4 Detection (TA506389) Antibodies. Substrate used: full length HEK293 cells expressed recombinant protein of human CD163 (TP308557).

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Specifications

Product Data

• Clone Name: OTI3C4
• Applications: IF, IHC, LMNX, WB
• Recommended Dilution: WB 1:4000, IHC 1:500, IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CD163(NP_004235) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 125.3 kDa
• Gene Name: Homo sapiens CD163 molecule (CD163), transcript variant 1, mRNA.
• Database Link:
  NP_004235
  Entrez Gene 9332 Human
  UniProt ID Q86VB7
• Synonyms: M130; MM130; SCARI1

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Transmembrane