P cadherin (CDH3) Mouse Monoclonal Antibody [Clone ID: OTI2D5]

CAT#: CF506403

Carrier-free (BSA/glycerol-free) CDH3 mouse monoclonal antibody, clone OTI2D5 (formerly 2D5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDH3 (RC207346, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDH3. Positive lysates LY400683 (100ug) and LC400683 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CDH3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506403)

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-CDH3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506403)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CDH3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506403)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CDH3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506403)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CDH3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506403)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CDH3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506403)

• Immunofluorescent staining of HeLa cells using anti-CDH3 mouse monoclonal antibody (TA506403).

• Anti-CDH3 mouse monoclonal antibody (TA506403) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CDH3 (RC207346).

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Specifications

Product Data

• Clone Name: OTI2D5
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:4000, IHC 1:150, IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CDH3(NP_001784) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 88.8 kDa
• Gene Name: Homo sapiens cadherin 3 (CDH3), transcript variant 1, mRNA.
• Database Link:
  NP_001784
  Entrez Gene 1001 Human
  UniProt ID P22223
• Background: This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. This gene is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. In addition, aberrant expression of this protein is observed in cervical adenocarcinomas. Mutations in this gene have been associated with congential hypotrichosis with juvenile macular dystrophy. [provided by RefSeq, Jul 2008]
• Synonyms: CDHP; HJMD; PCAD

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs)