alpha Synuclein (SNCA) Mouse Monoclonal Antibody [Clone ID: OTI2F6]

CAT#: CF506533

Carrier-free (BSA/glycerol-free) SNCA mouse monoclonal antibody, clone OTI2F6 (formerly 2F6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SNCA (RC210606, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SNCA. Positive lysates LY400127 (100ug) and LC400127 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SNCA mouse monoclonal antibody. (TA506533) (1:150).

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  Immunohistochemical staining of paraffin-embedded Human adult brain tissue within the normal limits using anti-SNCA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA506533) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum within the normal limits using anti-SNCA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA506533) (1:500)

Specifications

Product Data

• Clone Name: OTI2F6
• Applications: IHC, WB
• Recommended Dilution: WB 1:4000, IHC: 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SNCA(NP_000336) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 14.3 kDa
• Gene Name: Homo sapiens synuclein alpha (SNCA), transcript variant 1, mRNA.
• Database Link:
  NP_000336
  Entrez Gene 20617 MouseEntrez Gene 29219 RatEntrez Gene 6622 Human
  UniProt ID P37840
• Synonyms: NACP; PARK1; PARK4; PD1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Parkinson's disease