CD45 (PTPRC) Mouse Monoclonal Antibody [Clone ID: OTI1F6]

CAT#: CF506534

Carrier-free (BSA/glycerol-free) PTPRC mouse monoclonal antibody, clone OTI1F6 (formerly 1F6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTPRC (RC216590, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTPRC. Positive lysates LY401003 (100ug) and LC401003 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PTPRC monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PTPRC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506534)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PTPRC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506534)

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-PTPRC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506534)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PTPRC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506534)

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Specifications

Product Data

• Clone Name: OTI1F6
• Applications: IHC, WB
• Recommended Dilution: WB 1:400~4000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PTPRC(NP_002829) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 147.1 kDa
• Gene Name: Homo sapiens protein tyrosine phosphatase receptor type C (PTPRC), transcript variant 1, mRNA.
• Database Link:
  NP_002829
  Entrez Gene 5788 Human
  UniProt ID P08575
• Synonyms: B220; CD45; CD45R; GP180; L-CA; LCA; LY5; T200

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Phosphatase, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), Fc gamma R-mediated phagocytosis, Primary immunodeficiency, T cell receptor signaling pathway