Tyrosine Hydroxylase (TH) Mouse Monoclonal Antibody [Clone ID: OTI1A12]

CAT#: CF506540

Carrier-free (BSA/glycerol-free) TH mouse monoclonal antibody, clone OTI1A12 (formerly 1A12)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TH (RC211218, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TH. Positive lysates LY424777 (100ug) and LC424777 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TH mouse monoclonal antibody. (TA506540)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-TH mouse monoclonal antibody. (TA506540)

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  Anti-TH mouse monoclonal antibody (TA506540) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TH (RC211218).

Specifications

Product Data

• Clone Name: OTI1A12
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:4000, IF 1:100, IHC: 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TH(NP_000351) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 55.4 kDa
• Gene Name: Homo sapiens tyrosine hydroxylase (TH), transcript variant 2, mRNA.
• Database Link:
  NP_000351
  Entrez Gene 21823 MouseEntrez Gene 25085 RatEntrez Gene 7054 Human
  UniProt ID P07101
• Background: The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jul 2008]
• Synonyms: DYT5b; DYT14; TYH

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Parkinson's disease, Tyrosine metabolism