CD45 (PTPRC) Mouse Monoclonal Antibody [Clone ID: OTI3C8]

CAT#: CF506566

Carrier-free (BSA/glycerol-free) PTPRC mouse monoclonal antibody,clone OTI3C8

Product Images

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PTPRC monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  HEK293T cells lysate (5 ug, left lane) and full length human recombinant protein of human PTPRC (NP_002829) produced in HEK293T cell (5 ug, right lane)were separated by SDS-PAGE and immunoblotted with anti-PTPRC.

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-PTPRC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506566)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PTPRC mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA506566) (1:4000)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PTPRC mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA506566) (1:4000)

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  Immunofluorescent staining of HeLa cells using anti-PTPRC mouse monoclonal antibody (TA506566).

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Specifications

Product Data

• Clone Name: OTI3C8
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:400~4000, IHC 1:500, IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PTPRC(NP_002829) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 147.1 kDa
• Gene Name: Homo sapiens protein tyrosine phosphatase receptor type C (PTPRC), transcript variant 1, mRNA.
• Database Link:
  NP_002829
  Entrez Gene 5788 Human
  UniProt ID P08575
• Synonyms: B220; CD45; CD45R; GP180; L-CA; LCA; LY5; T200

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Phosphatase, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), Fc gamma R-mediated phagocytosis, Primary immunodeficiency, T cell receptor signaling pathway