TGF beta 1 (TGFB1) Mouse Monoclonal Antibody [Clone ID: OTI3B6]

CAT#: CF506583

Carrier-free (BSA/glycerol-free) TGFB1 mouse monoclonal antibody, clone OTI3B6 (formerly 3B6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TGFB1 (RC200973, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TGFB1 (1:2000).

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  Western blot analysis of extracts (35ug) from 5 different cell lines by using anti-TGFB1 monoclonal antibody (1:500).

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  Anti-TGFB1 mouse monoclonal antibody (TA506583) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TGFB1 (RC200973) (1:100).

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  Immunofluorescent staining of HeLa cells using anti-TGFB1 mouse monoclonal antibody (TA506583) (1:100).

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  Flow cytometric Analysis of permeabilized Hela cells, using anti-TGFB1 antibody (TA506583), (Red), compared to an IgG isotype control, (green), and negative control (PBS), (Blue) (1:100).

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  Flow cytometric Analysis of permeabilized HCT116 cells, using anti-TGFB1 antibody (TA506583), (Red), compared to an IgG isotype control, (green), and negative control (PBS), (Blue) (1:100).

• Flow cytometric Analysis of permeabilized SF295 cells, using anti-TGFB1 antibody (TA506583), (Red), compared to an IgG isotype control, (green), and negative control (PBS), (Blue) (1:100).

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Specifications

Product Data

• Clone Name: OTI3B6
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF: 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TGFB1(NP_000651) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 28.4 kDa
• Gene Name: Homo sapiens transforming growth factor beta 1 (TGFB1), mRNA.
• Database Link:
  NP_000651
  Entrez Gene 21803 MouseEntrez Gene 59086 RatEntrez Gene 7040 Human
  UniProt ID P01137
• Synonyms: CED; DPD1; LAP; TGFB; TGFbeta

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Transcription Factors
• Protein Pathways: Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Cytokine-cytokine receptor interaction, Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM), MAPK signaling pathway, Pancreatic cancer, Pathways in cancer, Renal cell carcinoma, TGF-beta signaling pathway