TUBB1 Mouse Monoclonal Antibody [Clone ID: OTI1G5]

CAT#: CF506622

Carrier-free (BSA/glycerol-free) TUBB1 mouse monoclonal antibody,clone OTI1G5

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TUBB1 (RC207459, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TUBB1. Positive lysates LY410723 (100ug) and LC410723 (20ug) can be purchased separately from OriGene.

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  Anti-TUBB1 mouse monoclonal antibody (TA506622) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TUBB1 (RC207459).

Specifications

Product Data

• Clone Name: OTI1G5
• Applications: IF, WB
• Recommended Dilution: WB 1:4000, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TUBB1(NP_110400) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 50.1 kDa
• Gene Name: Homo sapiens tubulin beta 1 class VI (TUBB1), mRNA.
• Database Link:
  NP_110400
  Entrez Gene 545486 MouseEntrez Gene 679312 RatEntrez Gene 81027 Human
  UniProt ID Q9H4B7
• Synonyms: beta 1; beta tubulin 1; class VI; dJ543J19.4; OTTHUMP00000031411; tubulin

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Gap junction, Pathogenic Escherichia coli infection