TUBB2B Mouse Monoclonal Antibody [Clone ID: OTI0C3]

CAT#: CF506623

Carrier-free (BSA/glycerol-free) TUBB2B mouse monoclonal antibody, clone OTI0C3 (formerly 0C3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TUBB2B (RC209075, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TUBB2B. Positive lysates LY405880 (100ug) and LC405880 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TUBB2B monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TUBB2B mouse monoclonal antibody. (TA506623)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TUBB2B mouse monoclonal antibody. (TA506623)

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  Anti-TUBB2B mouse monoclonal antibody (TA506623) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TUBB2B (RC209075).

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  Immunofluorescent staining of HeLa cells using anti-TUBB2B mouse monoclonal antibody (TA506623).

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Specifications

Product Data

• Clone Name: OTI0C3
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:400-1:2000, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TUBB2B(NP_821080) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 49.8 kDa
• Gene Name: Homo sapiens tubulin beta 2B class IIb (TUBB2B), mRNA.
• Database Link:
  NP_821080
  Entrez Gene 73710 MouseEntrez Gene 291081 RatEntrez Gene 347733 Human
  UniProt ID Q9BVA1
• Synonyms: bA506K6.1; PMGYSA

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Gap junction, Pathogenic Escherichia coli infection