EPCAM Mouse Monoclonal Antibody [Clone ID: OTI1C1]

CAT#: CF506629

Carrier-free (BSA/glycerol-free) EPCAM mouse monoclonal antibody,clone OTI1C1

Product Images

Anti-EPCAM mouse monoclonal antibody (TA506629) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY EPCAM (RC201989).

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EPCAM (RC201989, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EPCAM. Positive lysates LY400847 (100ug) and LC400847 (20ug) can be purchased separately from OriGene.

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  Flow cytometric analysis of live HEK293 cells transfected with EPCAM ORF clone (Cat# RC201989), using anti-EPCAM antibody (TA506629 at 1:100, Red), compared to IgG1 isotype control (Black).

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  Flow cytometric analysis of live OVCAR-4 cells, using anti-EPCAM antibody (TA506629 at 1:100, Red), compared to IgG1 isotype control (Black).

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  Flow cytometric analysis of live HT29 cells, using anti-EPCAM antibody (TA506629 at 1:100, Red), compared to IgG1 isotype control (Black).

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-EPCAM monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:400).

• Western blot analysis of extracts (10ug) from 1 cell line by using anti-EPCAM monoclonal antibody at 1:200.

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-EPCAM mouse monoclonal antibody at 1:500 dilution. (TA506629)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-EPCAM mouse monoclonal antibody at 1:500 dilution. (TA506629)

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Specifications

Product Data

• Clone Name: OTI1C1
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB: 1:400-1:1000, IHC: 1:150-1:500, IF: 1:100, Flow: 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human EPCAM(NP_002345) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 32.7 kDa
• Gene Name: Homo sapiens epithelial cell adhesion molecule (EPCAM), mRNA.
• Database Link:
  NP_002345
  Entrez Gene 4072 Human
  UniProt ID P16422
• Background: This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]
• Synonyms: 4; DIAR5; EGP-2; EGP40; EGP314; ESA; HNPCC8; KS1; KSA; M4S1; MIC18; MK-1; TACSTD1; TROP1

Reference Data

• Protein Families: ES Cell Differentiation/IPS, Transmembrane