FOXP1 Mouse Monoclonal Antibody [Clone ID: OTI4F1]

CAT#: CF506702

Carrier-free (BSA/glycerol-free) FOXP1 mouse monoclonal antibody, clone OTI4F1 (formerly 4F1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY FOXP1 (Cat# RC213862, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOXP1(Cat# TA506702). Positive lysates LY403191 (100ug) and LC403191 (20ug) can be purchased separately from OriGene.

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and FOXP1-Knockout 293T cells (KO, Cat# LC810347) were separated by SDS-PAGE and immunoblotted with anti-FOXP1 monoclonal antibody TA506702. Then the blotted membrane was stripped and reprobed with anti-b-actin antibody (TA811000) as a loading control (1:500).

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-FOXP1 mouse monoclonal antibody at 1:150 (TA506702). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-FOXP1 mouse monoclonal antibody at 1:150 (TA506702). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-FOXP1 mouse monoclonal antibody at 1:150 (TA506702). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-FOXP1 mouse monoclonal antibody at 1:150 (TA506702). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-FOXP1 mouse monoclonal antibody at 1:150 (TA506702). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-FOXP1 mouse monoclonal antibody at 1:150 (TA506702). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-FOXP1 mouse monoclonal antibody at 1:150 (TA506702). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-FOXP1 mouse monoclonal antibody at 1:150 (TA506702). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-FOXP1 mouse monoclonal antibody at 1:150 (TA506702). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-FOXP1 mouse monoclonal antibody at 1:150 (TA506702). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-FOXP1 mouse monoclonal antibody at 1:150 (TA506702). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunofluorescent staining of COS7 cells, transiently transfected with pCMV6-ENTRY FOXP1 (RC213862) by using Anti-FOXP1 mouse monoclonal antibody (TA506702) at 1:100 dilution.

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Specifications

Product Data

• Clone Name: OTI4F1
• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:1000-1:4000, IHC: 1:50-1:150, IF: 1:50-1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human FOXP1(NP_116071) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 75.1 kDa
• Gene Name: forkhead box P1
• Database Link:
  NP_116071
  Entrez Gene 27086 Human
  UniProt ID Q9H334
• Background: This gene belongs to subfamily P of the forkhead box (FOX) transcription factor family. Forkhead box transcription factors play important roles in the regulation of tissue- and cell type-specific gene transcription during both development and adulthood. Forkhead box P1 protein contains both DNA-binding- and protein-protein binding-domains. This gene may act as a tumor suppressor as it is lost in several tumor types and maps to a chromosomal region (3p14.1) reported to contain a tumor suppressor gene(s). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul
• Synonyms: 12CC4; hFKH1B; HSPC215; MFH; QRF1

Reference Data

• Protein Families: Transcription Factors