Oct4 (POU5F1) Mouse Monoclonal Antibody [Clone ID: OTI7H1]

CAT#: CF506798

Carrier-free (BSA/glycerol-free) POU5F1 mouse monoclonal antibody, clone OTI7H1 (formerly 7H1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POU5F1 (RC211998, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POU5F1. Positive lysates LY400950 (100ug) and LC400950 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-POU5F1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Western blot analysis of extracts (35ug) from 1 cell line lysate and 4 tissue lysates by using anti-POU5F1 monoclonal antibody (1:250).

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  Anti-POU5F1 mouse monoclonal antibody (TA506798) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY POU5F1 (RC211998) (1:100).

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  Immunofluorescent staining of CRL1973 cells using anti-POU5F1 mouse monoclonal antibody (TA506798) (1:100).

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Specifications

Product Data

• Clone Name: OTI7H1
• Applications: IF, WB
• Recommended Dilution: WB 1:200~1000, IF: 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human POU5F1(NP_002692) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 38.4 kDa
• Gene Name: Homo sapiens POU class 5 homeobox 1 (POU5F1), transcript variant 1, mRNA.
• Database Link:
  NP_002692
  Entrez Gene 18999 MouseEntrez Gene 294562 RatEntrez Gene 5460 Human
  UniProt ID Q01860
• Background: This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq, Mar 2010]
• Synonyms: Oct-3; Oct-4; OCT3; OCT4; OTF-3; OTF3; OTF4

Reference Data

• Protein Families: Adult stem cells, Cancer stem cells, Embryonic stem cells, Induced pluripotent stem cells, Stem cell - Pluripotency, Transcription Factors