IL1 alpha (IL1A) Mouse Monoclonal Antibody [Clone ID: OTI3C3]

CAT#: CF506853

Carrier-free (BSA/glycerol-free) IL1A mouse monoclonal antibody, clone OTI3C3 (formerly 3C3)

Product Images

Negative control HEK293T lysate (Left lane) or full length human recombinant protein of human IL1A (NP_000566) produced in HEK293T cell (Right lane). Equivalent amounts (5 ug per lane) were separated by SDS-PAGE and then immunoblotted with anti-IL1A.

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-IL1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506853)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-IL1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506853)

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-IL1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506853)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-IL1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506853)

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  Anti-IL1A mouse monoclonal antibody (TA506853) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IL1A (RC202084).

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Specifications

Product Data

• Clone Name: OTI3C3
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human IL1A(NP_000566) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens interleukin 1 alpha (IL1A), transcript variant 1, mRNA.
• Database Link:
  NP_000566
  Entrez Gene 3552 Human
  UniProt ID P01583
• Background: The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease. [provided by RefSeq, Jul 2008]
• Synonyms: IL-1A; IL1; IL1-ALPHA; IL1F1

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Apoptosis, Cytokine-cytokine receptor interaction, Graft-versus-host disease, Hematopoietic cell lineage, MAPK signaling pathway, Prion diseases, Type I diabetes mellitus