SERPINB3 Mouse Monoclonal Antibody [Clone ID: OTI1G1]

CAT#: CF506885

Carrier-free (BSA/glycerol-free) SERPINB3 mouse monoclonal antibody, clone OTI1G1 (formerly 1G1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SERPINB3 (RC202683, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SERPINB3. Positive lysates LY416321 (100ug) and LC416321 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SERPINB3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506885)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SERPINB3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506885)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SERPINB3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506885)

Specifications

Product Data

• Clone Name: OTI1G1
• Applications: IHC, WB
• Recommended Dilution: WB 1:4000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SERPINB3(NP_008850) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 44.4 kDa
• Gene Name: Homo sapiens serpin family B member 3 (SERPINB3), mRNA.
• Database Link:
  NP_008850
  Entrez Gene 6317 Human
  UniProt ID P29508
• Synonyms: HsT1196; SCC; SCCA-1; SCCA-PD; SCCA1; SSCA1; T4-A

Reference Data

• Protein Families: Druggable Genome, Secreted Protein