SCAMP2 Mouse Monoclonal Antibody [Clone ID: OTI2F3]

CAT#: CF506956

Carrier-free (BSA/glycerol-free) SCAMP2 mouse monoclonal antibody, clone OTI2F3 (formerly 2F3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SCAMP2 (RC200262, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SCAMP2. Positive lysates LY417127 (100ug) and LC417127 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SCAMP2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human). Dilution: 1:400

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  Anti-SCAMP2 mouse monoclonal antibody (TA506956) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SCAMP2 (RC200262).

Specifications

Product Data

• Clone Name: OTI2F3
• Applications: IF, WB
• Recommended Dilution: WB 1:4000, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SCAMP2(NP_005688) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 36.5 kDa
• Gene Name: Homo sapiens secretory carrier membrane protein 2 (SCAMP2), transcript variant 2, mRNA.
• Database Link:
  NP_005688
  Entrez Gene 10066 Human
  UniProt ID O15127
• Background: This gene product belongs to the SCAMP family of proteins which are secretory carrier membrane proteins. They function as carriers to the cell surface in post-golgi recycling pathways. Different family members are highly related products of distinct genes, and are usually expressed together. These findings suggest that the SCAMPs may function at the same site during vesicular transport rather than in separate pathways. [provided by RefSeq, Jul 2008]
• Synonyms: secretory carrier membrane protein 2

Reference Data

• Protein Families: Transmembrane