Hydroxysteroid (17 beta) Dehydrogenase 4 (HSD17B4) Mouse Monoclonal Antibody [Clone ID: OTI2C9]

CAT#: CF507075

Carrier-free (BSA/glycerol-free) HSD17B4 mouse monoclonal antibody, clone OTI2C9 (formerly 2C9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSD17B4 (RC200460, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSD17B4. Positive lysates LY424737 (100ug) and LC424737 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-HSD17B4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA507075)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-HSD17B4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA507075)

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  Anti-HSD17B4 mouse monoclonal antibody (TA507075) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HSD17B4 (RC200460).

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  Immunofluorescent staining of HeLa cells using anti-HSD17B4 mouse monoclonal antibody (TA507075).

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Specifications

Product Data

• Clone Name: OTI2C9
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:4000, IHC 1:150, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human HSD17B4(NP_000405) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 79.5 kDa
• Gene Name: Homo sapiens hydroxysteroid 17-beta dehydrogenase 4 (HSD17B4), transcript variant 2, mRNA.
• Database Link:
  NP_000405
  Entrez Gene 15488 MouseEntrez Gene 3295 Human
  UniProt ID P51659
• Background: The protein encoded by this gene is a bifunctional enzyme that is involved in the peroxisomal beta-oxidation pathway for fatty acids. It also acts as a catalyst for the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids. Defects in this gene that affect the peroxisomal fatty acid beta-oxidation activity are a cause of D-bifunctional protein deficiency (DBPD). An apparent pseudogene of this gene is present on chromosome 8. [provided by RefSeq, Jul 2008]
• Synonyms: DBP; MFE-2; MPF-2; PRLTS1; SDR8C1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Primary bile acid biosynthesis