MT (MCAT) Mouse Monoclonal Antibody [Clone ID: OTI2H4]

CAT#: CF507080

Carrier-free (glycerol/BSA-free) MCAT mouse monoclonal antibody, clone OTI2H4 (formerly 2H4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCAT (RC207943, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCAT. Positive lysates LY406620 (100ug) and LC406620 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI2H4
• Applications: WB
• Recommended Dilution: WB 1:400~4000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MCAT(NP_775738) produced in HEK293T cell
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 40.6 kDa
• Gene Name: Homo sapiens malonyl-CoA-acyl carrier protein transacylase (MCAT), transcript variant 1, mRNA.
• Database Link:
  NP_775738
  Entrez Gene 27349 Human
  UniProt ID Q8IVS2
• Background: The protein encoded by this gene is found exclusively in the mitochondrion, where it catalyzes the transfer of a malonyl group from malonyl-CoA to the mitochondrial acyl carrier protein. The encoded protein may be part of a fatty acid synthase complex that is more like the type II prokaryotic and plastid complexes rather than the type I human cytosolic complex. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2012]
• Synonyms: fabD; FASN2C; MCT; MT; NET62

Reference Data

• Protein Pathways: Fatty acid biosynthesis, Metabolic pathways