PD L1 (CD274) Mouse Monoclonal Antibody [Clone ID: OTI4D4]

CAT#: CF507099

Carrier-free (BSA/glycerol-free) CD274 mouse monoclonal antibody, clone OTI4D4 (formerly 4D4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD274 (RC213071, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD274. Positive lysates LY415473 (100ug) and LC415473 (20ug) can be purchased separately from OriGene.

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  PD-L1 ELISA with 9E12 Capture (TA808771) and 4D4 Detection (TA507099) Antibodies. Substrate used: Recombinant Human PD-L1 (TP700201)

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  PD-L1 Luminex ELISA with 9E12 Capture (TA808771) and 4D4 Detection (TA507099) Antibodies. Substrate used: Recombinant Human PD-L1 (TP700201)

Specifications

Product Data

• Clone Name: OTI4D4
• Applications: ELISA, LMNX, WB
• Recommended Dilution: WB 1:4000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CD274(NP_054862) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 31 kDa
• Gene Name: Homo sapiens CD274 molecule (CD274), transcript variant 1, mRNA.
• Database Link:
  NP_054862
  Entrez Gene 29126 Human
  UniProt ID Q9NZQ7
• Synonyms: B7-H; B7H1; PD-L1; PDCD1L1; PDCD1LG1; PDL1

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs)