SCARB1 Mouse Monoclonal Antibody [Clone ID: OTI1F3]

CAT#: CF507135

Carrier-free (BSA/glycerol-free) SCARB1 mouse monoclonal antibody, clone OTI1F3 (formerly 1F3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SCARB1 (RC210264, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SCARB1. Positive lysates LY401684 (100ug) and LC401684 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SCARB1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human). Dilution: 1:400

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-SCARB1 mouse monoclonal antibody. (TA507135)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-SCARB1 mouse monoclonal antibody. (TA507135)

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  Anti-SCARB1 mouse monoclonal antibody (TA507135) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SCARB1 (RC210264).

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Specifications

Product Data

• Clone Name: OTI1F3
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:4000, IF: 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SCARB1(NP_005496) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 56.8 kDa
• Gene Name: Homo sapiens scavenger receptor class B member 1 (SCARB1), transcript variant 1, mRNA.
• Database Link:
  NP_005496
  Entrez Gene 949 Human
  UniProt ID Q8WTV0
• Background: The protein encoded by this gene is a plasma membrane receptor for high density lipoprotein cholesterol (HDL). The encoded protein mediates cholesterol transfer to and from HDL. In addition, this protein is a receptor for hepatitis C virus glycoprotein E2. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar
• Synonyms: CD36L1; CLA-1; CLA1; HDLQTL6; SR-BI; SRB1

Reference Data

• Protein Families: Druggable Genome, Transmembrane