Mannan Binding Lectin (MBL2) Mouse Monoclonal Antibody [Clone ID: OTI1A12]

CAT#: CF507147

Carrier-free (BSA/glycerol-free) MBL2 mouse monoclonal antibody, clone OTI1A12 (formerly 1A12)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MBL2 (RC210143, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MBL2. Positive lysates LY424849 (100ug) and LC424849 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-MBL2 mouse monoclonal antibody (TA507147) at 1:150 dilution.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-MBL2 mouse monoclonal antibody (TA507147) at 1:150 dilution.

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  Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MBL2 (RC210143) using Anti-MBL2 mouse monoclonal antibody (TA507147) at 1:100 dilution.

Specifications

Product Data

• Clone Name: OTI1A12
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:4000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MBL2(NP_000233) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 24 kDa
• Gene Name: Homo sapiens mannose binding lectin 2 (MBL2), transcript variant 1, mRNA.
• Database Link:
  NP_000233
  Entrez Gene 4153 Human
  UniProt ID P11226
• Background: This gene encodes the soluble mannose-binding lectin or mannose-binding protein found in serum. The protein encoded belongs to the collectin family and is an important element in the innate immune system. The protein recognizes mannose and N-acetylglucosamine on many microorganisms, and is capable of activating the classical complement pathway. Deficiencies of this gene have been associated with susceptibility to autoimmune and infectious diseases. [provided by RefSeq, Jul 2008]
• Synonyms: COLEC1; HSMBPC; MBL; MBL2D; MBP; MBP-C; MBP1; MBPD

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Complement and coagulation cascades