MINPP1 Mouse Monoclonal Antibody [Clone ID: OTI1B2]

CAT#: CF507234

Carrier-free (BSA/glycerol-free) MINPP1 mouse monoclonal antibody, clone OTI1B2 (formerly 1B2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MINPP1 (RC207581, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MINPP1. Positive lysates LY417667 (100ug) and LC417667 (20ug) can be purchased separately from OriGene.

• 

  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-MINPP1 mouse monoclonal antibody (TA507234) at 1:150 dilution.

• 

  Anti-MINPP1 mouse monoclonal antibody (TA507234) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MINPP1 (RC207581).

Specifications

Product Data

• Clone Name: OTI1B2
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:4000, IF 1:100, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MINPP1(NP_004888) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 54.9 kDa
• Gene Name: Homo sapiens multiple inositol-polyphosphate phosphatase 1 (MINPP1), transcript variant 1, mRNA.
• Database Link:
  NP_004888
  Entrez Gene 9562 Human
  UniProt ID Q9UNW1
• Background: This gene encodes multiple inositol polyphosphate phosphatase; an enzyme that removes 3-phosphate from inositol phosphate substrates. It is the only enzyme known to hydrolzye inositol pentakisphosphate and inositol hexakisphosphate. This enzyme also converts 2,3 bisphosphoglycerate (2,3-BPG) to 2-phosphoglycerate; an activity formerly thought to be exclusive to 2,3-BPG synthase/2-phosphatase (BPGM) in the Rapoport-Luebering shunt of the glycolytic pathway. [provided by RefSeq, Sep 2009]
• Synonyms: HIPER1; MINPP2; MIPP

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Inositol phosphate metabolism