Albumin (ALB) Mouse Monoclonal Antibody [Clone ID: OTI5H2]

CAT#: CF507290

Carrier-free (BSA/glycerol-free) ALB mouse monoclonal antibody, clone OTI5H2 (formerly 5H2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALB (RC205943, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALB. Positive lysates LY424677 (100ug) and LC424677 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 1 cell line by using anti-ALB monoclonal antibody (1:200).

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  Western blot analysis of extracts (35ug) from 2 cell lines and 2 tissues lysates by using anti-ALB monoclonal antibody (1:1000).

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  Immunofluorescent staining of HepG2 cells using anti-ALB antibody (TA507290/green, upper left; DAPI/blue, lower left; MERGED, upper right) or Isotype control (MERGED, lower right) (1:100).

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  Immunofluorescent staining of Huh7 cells using anti-ALB antibody (TA507290/green, upper left; DAPI/blue, lower left; MERGED, upper right) or Isotype control (MERGED, lower right) (1:100).

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Specifications

Product Data

• Clone Name: OTI5H2
• Applications: IF, WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 19-609 of human ALB (NP_000468) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 67.2 kDa
• Gene Name: Homo sapiens albumin (ALB), mRNA.
• Database Link:
  NP_000468
  Entrez Gene 213 Human
  UniProt ID P02768
• Background: Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin. [provided by RefSeq, Jul
• Synonyms: HSA; PRO0883; PRO0903; PRO1341

Reference Data

• Protein Families: Secreted Protein